Figure 2.

md130 mutant products. (A) The sequence of the wild-type unc-64 gene in the region of the md130 mutation. The G to A md130 mutation at the splice-donor site of the sixth intron is shown. Alternative, downstream splice-donor sites used to produce the cloned md130 cDNA species are underlined. (B) Protein products predicted from the sequence of md130 cDNAs. Some wild-type UNC-64(+) product is predicted. One mutant product, md130A, would be made from mRNA spliced at the upstream alternative donor site; md130B would be produced from mRNA spliced at the downstream site or by unspliced mRNA.