Table 1.

Transformation rescue of mdl30

Injected plasmids	GFP expression	Dispersal index†		Fraction aldicarb-resistant animals‡
		With 3–4% (vol/vol) isoflurane	With no isoflurane
Syntaxin + GFP marker	−	0.45  ± 0.04	0.63  ± 0.04	0.78  ± 0.06
Syntaxin + GFP marker	+	0.13  ± 0.02*	0.98  ± 0.01*	0.17  ± 0.02*
GFP marker	−	0.59  ± 0.03	0.66  ± 0.11	ND
GFP marker	+	0.48  ± 0.06	0.78  ± 0.06	ND

The syntaxin plasmid pTX20 contains a full-length genomic syntaxin insert (21). The GFP transformation marker plasmid pMR1 contains a myosin promoter-driven GFP insert (21). 

^†Dispersal index (mean ± SEM) for a given GFP expression of independently transformed lines (n = 10 for syntaxin + GFP; n = 4 for GFP only). For isoflurane sensitivity determinations, each line was tested at a single concentration, ranging from 3–4% (vol/vol). 

^‡Fraction of animals moving at 0.5 mM aldicarb. 

^*, P < 0.01 GFP(+) vs. GFP(−); ND, not determined.