Table 2.
Effect of mutations in presynaptic proteins on VA sensitivity
| Strain | Mutant gene | Halothane EC50, % (vol/vol)† | Isoflurane EC50, % (vol/vol)† | Aldicarb EC80–95, mM‡ | Dispersal index (no VA)§ |
|---|---|---|---|---|---|
| N2 | None (wild type) | 0.45 ± 0.02 | 0.74 ± 0.02 | 0.5 | 0.88 ± 0.01 |
| unc-64(md130) | Syntaxin (ref. 21) | 1.29 ± 0.12* | 4.36 ± 0.36* | 1.5 | 0.63 ± 0.03 |
| unc-64(js21) | Syntaxin (ref. 21) | 0.17 ± 0.03* | 0.13 ± 0.04* | 1.5 | 0.64 ± 0.05 |
| unc-64(md1259) | Syntaxin (ref. 21) | 0.17 ± 0.10* | 0.18 ± 0.02* | 1.5 | 0.40 ± 0.07 |
| snb-1(md247) | Synaptobrevin (ref. 19) | 0.11 ± 0.02* | 0.18 ± 0.08* | 2 | 0.55 ± 0.1 |
| ric-4(js20) | SNAP-25 (ref. 13) | 0.22 ± 0.06* | 0.52 ± 0.05 | 1 | 0.62 ± 0.06 |
| rab-3(js49) | rab3 (ref. 18) | 0.37 ± 0.02 | 0.67 ± 0.05 | 1 | 0.78 ± 0.03 |
| aex-3(y255) | rab3 EF (refs. 17 and 32) | 0.41 ± 0.09 | 0.48 ± 0.05 | 1 | 0.89 ± 0.03 |
EF = nucleotide exchange factor. ∗, P < 0.01; different from N2.
†
Halothane and isoflurane EC50 values are estimated from at least eight concentration/response values (mean ± SEM).
‡
Aldicarb EC80–95 is the aldicarb millimolar concentration at which 80–95% of animals are paralyzed in the absence of anesthetic.
§
Values are mean ± SEM.