Figure 5.

Yeast Model

(A) Phenotypic analysis of the DMT1-4C strain (Δerv1) carrying either ERV1 or the erv1^R182H allele. The growth phenotype was that observed at 37°C. Equal amounts of serial dilutions of cells from exponentially grown cultures were spotted onto YNB medium supplemented with 2% glucose.

(B) Cytochrome spectra of cells grown on YNB medium supplemented with 2% glucose at 28°C and then shifted to 37°C overnight (A denotes absorbance).

(C) COX activity measured in the ERV1 and erv1^R182H strains.

(D) Petite frequency of Δerv1 strain transformed with wild-type ERV1 and the erv1^R182H mutant allele. COX activity and petite frequency determinations were performed in triplicate. The error bars represent SD.