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. 2008 Jul;43(1-3):126–134. doi: 10.1016/j.bone.2008.03.019

Table 3.

Deiodinase enzyme activities in osteoclasts

Primary rat monocyte − RANKL extracts 3 rats n = 2 in triplicate Primary rat osteoclast + RANKL extracts 3 rats n = 2 in triplicate RAW 264.7 3, 5, 7 day extracts and intact cells n = 2 in triplicate
D1 (fmol/min/mg) 0 0 0
D2 (fmol/min/mg) 0 0 0
D3 (fmol/min/mg) 1.13 1.0 0

Positive control adult human liver extracts deiodinated 100% of substrate in each 120 min D1 assay; COS-1 cell extracts transfected with human D2 possessed 12.06 fmol/min/mg D2 activity; ECC1 cell and human fetal liver extracts possessed D3 activities of 9.83 and 208 fmol/min/mg, respectively.