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. 1990 Sep;28(9):2094–2098. doi: 10.1128/jcm.28.9.2094-2098.1990

High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.

W R Butler 1, J O Kilburn 1
PMCID: PMC268109  PMID: 2229390

Abstract

Rapidly growing mycobacteria of clinical significance were identified by mycolic acids detected with high-performance liquid chromatography. Mycolic acids from whole cells were extracted, derivatized, and detected by a modified high-performance liquid chromatography procedure in less than 3 h. Use of an internal standard allowed differentiation of Mycobacterium chelonae and Mycobacterium fortuitum by comparison of relative retention times. Peak height ratios were used for subidentification of M. chelonae strains; however, M. fortuitum and Mycobacterium smegmatis could not be separated by this system.

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Selected References

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  1. Baess I. Deoxyribonucleic acid relatedness among species of rapidly growing mycobacteria. Acta Pathol Microbiol Immunol Scand B. 1982 Oct;90(5):371–375. doi: 10.1111/j.1699-0463.1982.tb00133.x. [DOI] [PubMed] [Google Scholar]
  2. Butler W. R., Kilburn J. O. Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids. J Clin Microbiol. 1988 Jan;26(1):50–53. doi: 10.1128/jcm.26.1.50-53.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casal M. J., Rodriguez F. C. In vitro susceptibility of Mycobacterium fortuitum and M. chelonei to sisomicin, gentamicin and tobramycin. Ann Microbiol (Paris) 1983 Nov-Dec;134B(3):451–454. [PubMed] [Google Scholar]
  4. Davidson L. A., Draper P., Minnikin D. E. Studies on the mycolic acids from the walls of Mycobacterium microti. J Gen Microbiol. 1982 Apr;128(4):823–828. doi: 10.1099/00221287-128-4-823. [DOI] [PubMed] [Google Scholar]
  5. Durst H. D., Milano M., Kikta E. J., Jr, Connelly S. A., Grushka E. Phenacyl esters of fatty acids via crown ether catalysts for enhanced ultraviolet detection in liquid chromatography. Anal Chem. 1975 Sep;47(11):1797–1801. doi: 10.1021/ac60361a025. [DOI] [PubMed] [Google Scholar]
  6. Goodfellow M., Minnikin D. E. Identification of mycobacterium chelonei by thin-layer chromatographic analysis of whole-organism methanolysates. Tubercle. 1981 Dec;62(4):285–287. doi: 10.1016/s0041-3879(81)80010-4. [DOI] [PubMed] [Google Scholar]
  7. Knisley C. V., Damato J. J., McClatchy J. K., Brennan P. J. Rapid and sensitive identification of Mycobacterium tuberculosis. J Clin Microbiol. 1985 Nov;22(5):761–767. doi: 10.1128/jcm.22.5.761-767.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lambert M. A., Moss C. W., Silcox V. A., Good R. C. Analysis of mycolic acid cleavage products and cellular fatty acids of Mycobacterium species by capillary gas chromatography. J Clin Microbiol. 1986 Apr;23(4):731–736. doi: 10.1128/jcm.23.4.731-736.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lévy-Frébault V., Daffé M., Goh K. S., Lanéelle M. A., Asselineau C., David H. L. Identification of Mycobacterium fortuitum and Mycobacterium chelonei. J Clin Microbiol. 1983 May;17(5):744–752. doi: 10.1128/jcm.17.5.744-752.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maliwan N., Reid R. W., Pliska S. R., Bird T. J., Zvetina J. R. Identifying Mycobacterium tuberculosis cultures by gas-liquid chromatography and a computer-aided pattern recognition model. J Clin Microbiol. 1988 Feb;26(2):182–187. doi: 10.1128/jcm.26.2.182-187.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Minnikin D. E., Dobson G., Goodfellow M., Draper P., Magnusson M. Quantitative comparison of the mycolic and fatty acid compositions of Mycobacterium leprae and Mycobacterium gordonae. J Gen Microbiol. 1985 Aug;131(8):2013–2021. doi: 10.1099/00221287-131-8-2013. [DOI] [PubMed] [Google Scholar]
  12. Minnikin D. E., Minnikin S. M., Goodfellow M., Stanford J. L. The mycolic acids of Mycobacterium chelonei. J Gen Microbiol. 1982 Apr;128(4):817–822. doi: 10.1099/00221287-128-4-817. [DOI] [PubMed] [Google Scholar]
  13. Minnikin D. E., Minnikin S. M., Hutchinson I. G., Goodfellow M., Grange J. M. Mycolic acid patterns of representative strains of Mycobacterium fortuitum, 'Myobacterium peregrinum' and Mycobacterium smegmatis. J Gen Microbiol. 1984 Feb;130(2):363–367. doi: 10.1099/00221287-130-2-363. [DOI] [PubMed] [Google Scholar]
  14. Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M., Magnusson M. Mycolic acid patterns of some species of Mycobacterium. Arch Microbiol. 1984 Oct;139(2-3):225–231. doi: 10.1007/BF00402005. [DOI] [PubMed] [Google Scholar]
  15. Morgan M. A. The radiometric detection and identification by gas liquid chromatography of mycobacteria from clinical specimens. Ann N Y Acad Sci. 1984;428:230–235. doi: 10.1111/j.1749-6632.1984.tb12298.x. [DOI] [PubMed] [Google Scholar]
  16. Moss C. W., Lambert M. A., Merwin W. H. Comparison of rapid methods for analysis of bacterial fatty acids. Appl Microbiol. 1974 Jul;28(1):80–85. doi: 10.1128/am.28.1.80-85.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moss C. W., Shinoda T., Samuels J. W. Determination of cellular fatty acid compositions of various yeasts by gas-liquid chromatography. J Clin Microbiol. 1982 Dec;16(6):1073–1079. doi: 10.1128/jcm.16.6.1073-1079.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reiner E., Kubica G. P. Predictive value of pyrolysis-gas-liquid chromatography in the differentiation of mycobacteria. Am Rev Respir Dis. 1969 Jan;99(1):42–49. doi: 10.1164/arrd.1969.99.1.42. [DOI] [PubMed] [Google Scholar]
  19. Silcox V. A., Good R. C., Floyd M. M. Identification of clinically significant Mycobacterium fortuitum complex isolates. J Clin Microbiol. 1981 Dec;14(6):686–691. doi: 10.1128/jcm.14.6.686-691.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Swenson J. M., Thornsberry C., Silcox V. A. Rapidly growing mycobacteria: testing of susceptibility to 34 antimicrobial agents by broth microdilution. Antimicrob Agents Chemother. 1982 Aug;22(2):186–192. doi: 10.1128/aac.22.2.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tisdall P. A., DeYoung D. R., Roberts G. D., Anhalt J. P. Identification of clinical isolates of mycobacteria with gas-liquid chromatography: a 10-month follow-up study. J Clin Microbiol. 1982 Aug;16(2):400–402. doi: 10.1128/jcm.16.2.400-402.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tisdall P. A., Roberts G. D., Anhalt J. P. Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone. J Clin Microbiol. 1979 Oct;10(4):506–514. doi: 10.1128/jcm.10.4.506-514.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tomiyasu I. Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J Bacteriol. 1982 Aug;151(2):828–837. doi: 10.1128/jb.151.2.828-837.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Toriyama S., Yano I., Masui M., Kusunose E., Kusunose M., Akimori N. Regulation of cell wall mycolic acid biosynthesis in acid-fast bacteria. I. Temperature-induced changes in mycolic acid molecular species and related compounds in Mycobacterium phlei. J Biochem. 1980 Jul;88(1):211–221. [PubMed] [Google Scholar]
  25. Toriyama S., Yano I., Masui M., Kusunose M., Kusunose E. Separation of C50--60 and C70--80 mycolic acid molecular species and their changes by growth temperatures in Mycobacterium phlei. FEBS Lett. 1978 Nov 1;95(1):111–115. doi: 10.1016/0014-5793(78)80063-5. [DOI] [PubMed] [Google Scholar]
  26. Wallace R. J., Jr, Nash D. R., Tsukamura M., Blacklock Z. M., Silcox V. A. Human disease due to Mycobacterium smegmatis. J Infect Dis. 1988 Jul;158(1):52–59. doi: 10.1093/infdis/158.1.52. [DOI] [PubMed] [Google Scholar]
  27. Wallace R. J., Jr, Swenson J. M., Silcox V. A., Good R. C., Tschen J. A., Stone M. S. Spectrum of disease due to rapidly growing mycobacteria. Rev Infect Dis. 1983 Jul-Aug;5(4):657–679. doi: 10.1093/clinids/5.4.657. [DOI] [PubMed] [Google Scholar]

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