Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans

. Author manuscript; available in PMC: 2009 May 13.

Published in final edited form as: Nat Protoc. 2008;3(4):698–709. doi: 10.1038/nprot.2008.38

Component	Amount per reaction (μl)	Final

Primer mix (stock 25 μM each)	1	1 μM each
Diluted ‘Input’ DNA	2	10× to 80× depending on the dilution used
Nuclease-free water	10.5
SYBR Green mix	12.5