| Component | Amount per reaction (μl) | Final |
| Primer mix (stock 25 μM each) | 1 | 1 μM each |
| Sample DNA (diluted) | 4 | 1× |
| Nuclease-free water | 7.5 | |
| SYBR Green mix | 12.5 | |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.