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SFRP2 gene primer sequences, annealing temperature and product size for MethyLight assays
| CpG status | Forward primer (5’-3’) | Reverse primer (5’-3’) | Annealing temperature (°C) | Product size (bp) |
| M | GGGTCGGAGTTTTTCGGAGTTGCGC | CCGCTCTCTTCGCTAAATACGACTCG | 62 | 138 |
| U | TTTTGGGTTGGAGTTTTTTGGAGTTGTGT | AACCCACTCTCTTCACTAAATACAACTCA | 50 | 145 |
M: Methylated; U: Unmethylated.
