Cell lines were treated and differentiated into neurons (A) and osteoblasts (B). (C) A thyroid stimulating hormone (TSH)-dependent rat cell line FRTL5 was dedifferentiated by induction of a chimeric RAS protein. Expression profiles for orthologous miRNA precursor clusters with 5% or more relative cloning frequency in at least one sample are depicted in a color-coded manner (see Figure 3 for details). Abbreviations: dcAMP, dibutyryl cAMP; FBS, fetal bovine serum; TPA, trans-phorbol ester; RA, retinoic acid; USSC, human unrestricted somatic stem cell; orth, indicates that the hsa-mir-470 cluster is orthologous to the rodent mir-463 clusters.