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. Author manuscript; available in PMC: 2009 May 13.
Published in final edited form as: J Virol Methods. 2007 Jun 11;145(1):14–21. doi: 10.1016/j.jviromet.2007.04.013

Fig. 3.

Fig. 3

Performance comparison of electroporation to TransIT®-mRNA Kit transfected cells using a luciferase expressing replicon. Huh7 cells were either electrorporated with 5 μg or transfected with 2 μg of the YF-R.luc2A-RP RNA using the TransIT-mRNA Transfection Kit. Twenty-four hours post-delivery, the cells were harvested, lysed and assayed for both Renilla luciferase activity and total protein concentration. The luciferase activities were then normalized to total protein to account for differences in sample cell number.