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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Clin Cancer Res. 2009 Apr 7;15(8):2808–2817. doi: 10.1158/1078-0432.CCR-08-1953

Figure 3. Apoptotic effect of mAbs on MM cell lines.

Figure 3

Figure 3

Induction of apoptosis was evaluated by flow cytometry determination of (A) hypodiploid DNA and (B) activated caspase-3. Cells were incubated with drugs alone, milatuzumab alone, or combinations of drug + milatuzumab with and without a second antibody for cross-linking for 48 h. For determination of hypodiploid DNA, staining was done with propidium iodide. Changes in the intracellular levels of cleaved caspase-3 were measured using FITC–conjugated rabbit anti-activated caspase-3. Analyses were performed on the FACSCalibur. ○, solid line, drug only; △, dashed line, drug + milatuzumab; □, dotted line, drug + GAH; ●, solid line, drug + milatuzumab + GAH. Error bars represent standard deviations of 3 replicates, and at some points are hidden by the symbol.