Table 4. Summary of Preferential Interaction Coefficients for α-Chymotrypsinogen A as Measured by VPO^a.

cosolute	Ω3	Γμ1	Γμ3b	Γμ1 μ3
argHCl	(1.676 ± 0.001) - (0.514 ± 0.004)m3	(-2.8 ± 0.8) + (0.2 ± 5.8)m3 - (6.6 ± 8.1)m32	(0.9 ± 0.7)[3] - (10.5 ± 2.5)[3]2	(0.5 ± 0.7)[3] - (10.4 ± 2.5)[3]2
gdnHCl	(1.736 ± 0.001) - (0.488 ± 0.002)m3	(-0.8 ± 0.6) + (4.1 ± 1.1)m3	(4.0 ± 1)[3]	(3.9 ± 1)[3]
glucose	(1.022 ± 0.001) - (0.001 ± 0.002)m3	(-4.5 ± 1.2) - (5.1 ± 0.8)m3	(-5.2 ± 0.5)[3]	(-5.8 ± 0.5)[3]
glycerol	(0.999 ± 0.001) - (0.002 ± 0.002)m3	(-3.7 ± 0.8) - (13.6 ± 1.2)m3	(-15.0 ± 0.7)[3]	(-15.3 ± 0.7)[3]
urea	(0.902 ± 0.001) - (0.015 ± 0.001)m3	(-6.6 ± 0.8) + (8.8 ± 1.0)m3	(9.4 ± 1)[3]	(9.1 ± 1)[3]

It should be noted that [3] represents the molar concentration of the cosolute.

^aThe base buffer was 20 mM Na-Citrate, pH 3.5 and aCgn concentration held constant at 1.7 mM.

^bThe values represented are an average of the separate approximations computed by eqs 15 and 18.