Table 1.
Mutant | Sequence alterations | Symptoms on C. quinoac | Virion Properties |
||
---|---|---|---|---|---|
Yield (mg/g) | Size (nm) | Electophoretic Mobilityd | |||
Wild Type | Wild Type | CLL; SM | 5 | 28 | Normal |
Δ7aaa | N-41KAIKAIA47 deleted | CLL; no systemic infection | 0.4 | 28/26 | Faster |
Δl7aa-agrob | N-41KAIKAIA47 deleted | CLL; no systemic infection | 1.5 | 28/26 | Faster |
Δ7aa infection in plants was mediated through in vitro synthesized transcript inoculation.
Δ7aa infection in plants was mediated through agroinfiltration.
CLL, chlorotic local lesions; SM, systemic mottling
Electrophoretic mobility was carried out in 1% agarose gels prepared and electrphoresed in TAE buffer prior to staining with ethidium bromide.