Table 5.
mRNA expression in rhesus macaque CCs and GCs
| Gene Symbol |
Detection in CCs* |
Detection in GCs*
|
||||||
|---|---|---|---|---|---|---|---|---|
|
No. positive samples |
IVM/VVM† | P value† | No. positive samples
|
IVM/VVM† | P value† | |||
| CC-IVM | CC-VVM | GC-IVM | GC-VVM | |||||
| EGFR | 5/5 | 9/9 | 0.13 | 0.01 | 5/5 | 6/6 | 0.43 | 0.07 |
| EGF | 0/5 | 0/9 | ND | NA | 0/5 | 0/6 | ND | NA |
| AREG | 2/5 | 9/9 | Qual | NA | 5/5 | 6/6 | 6.36E-04 | 2.00E-03 |
| BTC | 5/5 | 9/9 | 0.11 | 0.01 | 5/5 | 6/6 | 0.17 | 0.04 |
| EREG | 4/5 | 9/9 | 0.84 | 0.90 | 5/5 | 6/6 | 0.94 | 0.96 |
| TGFα | 0/5 | 0/9 | ND | NA | 0/5 | 1/6 | ND | NA |
| CCND1 | 4/5 | 9/9 | 0.62 | 0.73 | 5/5 | 6/6 | 0.05 | 0.08 |
| CCND2 | 5/5 | 9/9 | 1.56 | 0.48 | 5/5 | 6/6 | 4.21 | 0.22 |
| CCNE1 | 4/5 | 9/9 | 0.04 | 0.04 | 5/5 | 6/6 | 2.94 | 0.22 |
| CCNE2 | 5/5 | 9/9 | 0.05 | 0.02 | 5/5 | 6/6 | 0.68 | 0.68 |
| CDK2 | 4/5 | 9/9 | 0.17 | 0.15 | 5/5 | 6/6 | 0.39 | 0.34 |
| CDK4 | 4/5 | 9/9 | 2.11 | 0.39 | 5/5 | 6/6 | 0.58 | 0.23 |
| CDK6 | 4/5 | 9/9 | 2.02 | 0.59 | 5/5 | 6/6 | 2.48 | 0.07 |
| POLA1 | 2/5 | 9/9 | Qual | NA | 5/5 | 6/6 | 0.80 | 0.85 |
CC, cumulus cells; GC, granulosa cells.
*
A sample is called “not detected” when an exponential phase of amplification does not begin before the 40th cycle of qPCR reaction.
†
IVM/VVM ratio and P value calculations were performed using groups with >3 detections of 5 samples and the samples called “no detection” were excluded from calculations. IVM:VVM <1.0 equals higher levels in vivo, whereas >1.0 equals lower levels in vivo. Qual, qualitative difference as judged by failure to detect signal in >3 replicates of 1 of the cell types. NA, not applicable. ND, not applicable due to no detection in >3 samples of either cell type.