Fig. 2.
Effects of diabetes on cardiac TXNIP expression. Wild-type mice were rendered diabetic by a single intraperitoneal injection of streptozotocin (STZ; 200 mg/kg) and euthanized 4 days later. Their hearts were harvested for immunoblotting. Bars represent mean fold change ± SE (n = 6 per group) in TXNIP (A) and cleaved caspase-3 (B) protein levels compared with control mice that received vehicle alone. Obese, insulin-resistant, and diabetic BTBRlepob/ob mice were euthanized at 12 wk of age and their cardiac TXNIP (C) and cleaved caspase-3 (D) expression was compared with age-matched lean control BTBRlep+/+ mice. Bars represent mean fold change ± SE (n = 3 per group).