Table 1.
The selectivities of the hairpins selected to bind to 1 or 2. Selectivities were determined by dividing the Kd determined for the other aminoglycoside by the Kd determined for the selected aminoglycoside
| Direct Assays | Competition Assays | |||||
|---|---|---|---|---|---|---|
| Kd for Selected Aminoglycoside (nM) | Kd for Other Aminoglycoside (nM) | Selectivity | Kd for Selected Aminoglycoside (nM) | Kd for Other Aminoglycoside (nM) | Selectivity | |
| KanHP | 680 ± 11 | 164 ± 22 | 5430 ± 396 | 33 | ||
| Mixturea | ||||||
| Kan HP1 | 235 ± 12 | ~4000 | 17 | >25000 | ||
| Kan HP2 | 314 ± 16 | 2800 ± 146 | 9 | >25000 | ||
| Kan HP3 | 373 ± 57 | 1800 ± 26 | 5 | >25000 | ||
| Kan HP5 | 429 ± 88 | 2200 ± 303 | 5 | >25000 | ||
| Nea HP | 1150 ± 166 | 275 ± 53 | ~3000 | ~11 | ||
| Mixturea | ||||||
| Nea HP1 | 135 ± 13 | ~3000 | ~22 | 89 ± 22 | ~5000 | ~55 |
| Nea HP3 | 629 ± 73 | ~5000 | ~7 | 115 ± 11 | ~4000 | ~35 |
| Nea HP5 | 692 ± 22 | ~3000 | ~4 | 94 ± 14 | ~3000 | ~30 |
| Nea HP7 | 751 ± 97 | 2000 ± 272 | 3 | 91 ± 16 | ~7000 | ~70 |
a
These RNAs are the mixtures of hairpins that were harvested via 2DCS for binding 1 or 2.