Fig. 3.

After hypoxia or DT-3 treatment, less myocardin and more Elk-1 binds to the MHC promoter in chromatin immunoprecipitation (ChIP) assays. FPVSMC were treated with hypoxia, normoxia, or DT-3 for 1 h. ChIP assays on MHC genes were performed on cross-linked chromatin in the treated FPVSMC with antibodies against myocardin or Elk-1 as described in materials and methods. DNA was analyzed by real-time PCR. A: data from ChIP on Elk-1. B: data from ChIP on myocardin. Data are generated from 3 independent experiments and expressed as means ± SE of 3 independent experiments. *P < 0.05 compared with the cells exposed to normoxia (expressed as 100%).