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. 2009 Feb 27;296(5):L780–L789. doi: 10.1152/ajplung.90295.2008

Fig. 8.

Fig. 8.

The effect of PKG on the expression of myocardin and Elk-1. A and B: inhibition of PKG by PKG siRNA transfection in FPVSMC downregulates myocardin but upregulates SRF and Elk-1. FPVSMC were transfected with PKG siRNA, and the protein expression of myocardin, SRF, and Elk-1 was measured by Western blot. Data are expressed as means ± SE of 4 independent experiments. *P < 0.05 compared with the cells transfected with control siRNA (expressed as 100%). C–E: overexpression of PKG in FPVSMC reverses hypoxia-induced downregulation of myocardin and MHC. FPVSMC were transfected with PKG tagged with GFP plasmid DNA or control vector and treated with hypoxia for 24 h after transfection, and the protein expression of myocardin, SRF, and Elk-1 was measured by Western blot. Blots are representatives from 3 independent experiments. C: representative Western blots. D: densitometric data of MHC. E: densitometric data of myocardin. Data are expressed as means ± SE of 4 independent experiments. *P < 0.05 compared with the cells transfected with control siRNA (expressed as 100%).