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Overexpression of PU.1, but not of PU.1S148A, supports L-PGDS expression in macrophages. RAW 264.7 cells were transfected with a host vector plasmid (lane 1) or a plasmid encoding PU.1 (lane 2) or PU.1S148A (lane 3). Total cell lysate of transfected cells was analyzed by Western blotting for L-PGDS, H-PGDS, and actin as internal controls.
