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Controlled perfusion from the early S1 segment at 20 nl/min with late proximal collection. Four collections were made from each nephron. The first 2 collections were made during perfusion with placebo and the last 2 with MK801 (1 μM). MK801 suppressed reabsorption while stability of repeated collections serves as time control (*P < 0.001 for effect of MK801 by repeated-measures ANOVA; n = 18 nephrons in 5 rats).
