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A: effect of the nitric oxide (NO) synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME) on Arg-induced inhibition of net O2− production by thick ascending limbs in the absence and presence of luminal flow. B: effect of l-NAME on Arg's effect on flow-stimulated net O2− production. *P < 0.002; n = 5 vs. Arg alone. Tubules were perfused at 20 nl/min.
