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Effect of MBG on phosphorylated Fli-1 in renal fibroblasts. A: immunoblot against phosphoserine in nuclear extracts immunoprecipitated with polyclonal antibody against Fli-1; representative blot is shown at top and quantification of n = 4 determinations is shown at bottom. B: 32P autoradiograph data obtained from cells treated with 32P-labeled phosphate for 2 h and MBG for 15 min. Top: representative autoradiograph. Bottom: quantification of n = 5 experiments. *P < 0.05 vs. control.
