Fig. 7.

The A563T variation was capable of increasing TRPV5-mediated uptake when present in a single allele and/or with other nonsynonymous SNP variations. A: the A563T variation increased TRPV5-mediated Ca²⁺ uptake in the presence of the R154H variation. The TRPV5 proteins encoded by 2 cRNA species mimicking the mRNAs derived from 2 identical or different alleles are shown using 2 adjacent vertical lines under the x-axis. Open, gray, and filled circles represent A8V, R154H, and A563T variation, respectively. The 2 cRNA species indicated in different groups were coinjected at an equal amount (6.25 ng/oocyte) to mimic the TRPV5 mRNA transcripts derived from the 2 alleles with different forms of variations in TRPV5. Data from 5 independent experiments are expressed as means ± SE. B: the A563T variation was capable of increasing TRPV5-mediated Ca²⁺ influx in the presence of A8V variation. Data from 3 independent experiments are expressed as means ± SE. *P <0.01 vs. control group (TRPV5). #P <0.01 vs. * groups by Student's t-test.