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Sarcomere length in the WT and NEB-KO GAS at P1 either at slack length or stretched by +1 fiber length (n = 4 WT slack, 4 WT stretched, 4 NEB-KO slack, and 4 NEB-KO stretched). Fisher's PLSD test revealed no significant difference in sarcomere length at either slack or stretched conditions, indicating that deletion of NEB does not interfere with the ability of the GAS to distribute tensile strains among its sarcomeres.
