Fig. 1.

TP-mediated ROS generation. hASMC were treated with IBOP for 3 h (A) or pretreated with NADPH oxidase inhibitors (B) (DPI, 10 μM, or apocynin, 100 μM) for 30 min prior to IBOP (100 nM; 3 h). C: mASMC from wild-type (WT) mice or p47^−/− mice were treated with IBOP (1 μM) for 3 h. ROS generation was assessed by fluorescent spectrophotometry or microscopy. Data in A and B are fold over basal ± SE (n = 3). *** P < 0.001 with reference to (w.r.t.) control. Data in C are a representative experiment that was repeated with similar results.