Fig. 2.
Effect of TP activation on TP expression. TPα-HEK cells were treated with IBOP (A, C) or iPE2III (B, D) for 12 h. Alternatively, HEK 293 cells transiently transfected with 3xHAhTPα were treated with 100 nM IBOP for 1–12 h (E) or pretreated with vehicle or 1 μM SQ 29548 for 30 min (F) prior to 100 nM IBOP for 6 h. Cell lysates were resolved by SDS-PAGE and HAhTPα detected using an anti-HA antibody. A and B are representative Western blots. Untransfected wild-type (WT) HEK 293 cells, probed for the anti-HA antibody, are shown as a control for nonspecific antibody binding (left panel, A). C–F are mean fold over basal expression of the mature TP ± SEM from densitometric analysis normalized to β-actin (n = 3–5). * P < 0.05, ** P < 0.005, and *** P < 0.001 w.r.t. control unless otherwise indicated.