Fig. 4.

Activation of the TP does not alter TP transcription. TPα-HEK (A) or hASMC (B, C) were serum starved (24 h) before IBOP treatment. Total RNA was extracted and reverse transcribed into cDNA, and TP expression was examined by real-time PCR. Values were normalized to either β-actin or 18S levels and are expressed as the fold change compared with control (no IBOP). Data are mean ± SEM (n = 3–5). ns, nonsignificant w.r.t control.