Fig. 4.

WAY-362450 repression of diet-induced gene expression. A: Total liver RNA prepared from apoE^−/−, LDLR^−/−, apoE^−/−SHP^−/−, and LDLR^−/−SHP^−/− mice was analyzed for mRNA levels of CYP7A1 and CYP8B1 by real-time PCR. Expression values for all samples were normalized for expression of GAPDH. The expression observed in female apoE^−/− or LDLR^−/− mice was defined as 1.0 for each panel. Values are the mean ± SEM. B: Total RNA was prepared from ileum and analyzed as above for expression of ABCG5 and ABCA1. * P < 0.01 for effect of WAY-362450 on gene expression.