TABLE 2.

WAY-362450 regulation of gene expression

		Hepatic SHP mRNA			Ileum SHP mRNA			Hepatic BSEP mRNA			Ileum IBABP mRN
		Chowa	Western	Westerrn + 450	Chow	Western	Western+450	Chow	Western	Western+450	Chow	Western	Western+450
LDLR−/−	Female	1.00 ± 0.14	0.49 ± 0.05	0.82 ± 0.06c	1.00 ± 0.23	1.75 ± 0.58	5.68 ± 1.28c	1.00 ± 0.14b	0.84 ± 0.09	1.98 ± 0.19c	1.00 ± 0.24	0.59 ± 0.09	1.72 ± 0.19c
	Male	0.57 ± 0.15	0.50 ± 0.07	0.89 ± 0.05c	0.59 ± 0.17	0.66 ± 0.13	9.42 ± 1.21c	0.71 ± 0.08	0.68 ± 0.09	1.40 ± 0.23c	1.93 ± 0.32	1.51 ± 0.25	2.57 ± 0.41c
apoE−/−	Female	1.00 ± 0.24	0.99 ± 0.16	1.34 ± 0.11c	1.00 ± 0.19	4.17 ± 0.53	16.7 ± 0.77c	1.00 ± 0.17b	0.54 ± 0.11	1.53 ± 0.12c	1.00 ± 0.10	1.15 ± 0.18	2.75 ± 0.14c
	Male	0.37 ± 0.04	0.52 ± 0.07	1.45 ± 0.13c	0.37 ± 0.16	2.58 ± 0.31	19.5 ± 3.92c	0.52 ± 0.03	0.48 ± 0.04	2.48 ± 0.12c	0.89 ± 0.10	1.26 ± 0.40	3.50 ± 0.20c
LDLR−/−	Female	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0.97 ± 0.09	0.48 ± 0.04	1.08 ± 0.10c	1.23 ± 0.25	1.47 ± 0.32	2.19 ± 0.36c
SHP−/−	Male	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0.44 ± 0.08	0.32 ± 0.05	1.20 ± 0.12c	2.13 ± 0.47	1.79 ± 0.40	3.69 ± 0.32c
apoE−/−	Female	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	1.01 ± 0.10	0.51 ± 0.11	1.20 ± 0.04c	1.29 ± 0.04	1.25 ± 0.19	2.03 ± 0.45c
SHP−/−	Male	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	1.17 ± 0.09	0.62 ± 0.13	1.58 ± 0.21c	1.35 ± 0.14	1.19 ± 0.09	3.12 ± 0.13c

^aMice were fed a standard chow diet, a Western diet, or a Western diet with WAY-362450 as described in Fig. 2. At the conclusion of the study, hepatic total TG and cholesterol levels were determined.

^bExpression levels in female mice fed the chow diet were defined as 1.00 for LDLR^−/− and apoE^−/−, respectively.

^cP < 0.05 for effect of WAY-362450.