Fig. 5.

Effects of adenoviral-mediated expression of kinase-inactive PKC-ζ on hepatic IKKβ activity (A), hepatic nuclear levels of the p65RelA subunit of NFκB (B), and NFκB-dependent EMSA mobility shifts (B), in high-fat–fed (HFF) mice. As in Fig. 1, where indicated, HFF mice were injected IV with adenovirus vector (Vec) or adenovirus encoding KI-PKC-ζ (KI-ζ), and 5 days later (to allow time for expression), mice were either fasted overnight or fed, or fed and treated IM for 15 min with saline or insulin in saline. For comparisons in assays, chow-fed (low fat) mice were similarly examined. Values are mean ± SEM of (N) determinations and P values (as per ANOVA) depict comparisons of indicated groups. Representative immunoblot of nuclear levels of the p65/RelA subunit of NFκB, and a representative autoradiogram of NFκB-dependent EMSA mobility shifts (arrow indicates electrophoretic mobility of the NFκB-DNA complex as determined with the kit standard (B). F indicates the electrophoretic front, as well as relative changes in multiple samples in the indicated groups.