Fig. 6.

Time-dependent apoA-I dissociation from cells expressing ABCA1 or ABCA1-W590S. HEK/ABCA1-GFP and HEK/ABCA1-W590S-GFP cells were incubated with 5 μg/ml Alexa546-labeled apoA-I for 15 min at 37°C and further incubated with 25 μg/ml nonlabeled apoA-I for the indicated times at 27°C. The relative amount of bound apoA-I was calculated as described in Materials and Methods with respect to the amount of apoA-I bound to cells expressing wild-type ABCA1 at time 0. *P < 0.05, **P < 0.001, significantly different from the relative amount of apoA-I bound to HEK/ABCA1-GFP cells at each time point.