Fig. 7.

NaTC increases ABCA1 on the plasma membrane. A: HEK/ABCA1-GFP cells were incubated in the presence of 0.02% BSA, 0.02% BSA and 1 mM NaTC, or 0.02% BSA and 5 μg/ml apoA-I for 12 h. Cells were then treated with sulfo-NHS-biotin, and cell lysates were prepared. Biotinylated cell surface proteins were precipitated with avidin-agarose from 150 μg of cell lysates. Cell lysates (15 μg of protein) and precipitated surface proteins were separated, and ABCA1-GFP and vinculin (loading control) were detected with indicated antibodies. B: Western blots were analyzed using a Fujifilm LAS-3000 imaging system. Experiments were performed in triplicate, and the average values are represented with the SEM.