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. 2009 Jun;50(6):1195–1202. doi: 10.1194/jlr.M800630-JLR200

Fig. 2.

Fig. 2.

Data showing FADS2 action on 20:2n-6. A: Reconstructed ion chromatograms of FAME derived from 48 h incubation of 20:2n-6 with wt S. cerevisiae; B: S. cerevisiae transformed with empty vector; and C: S. cerevisiae transformed with FADS2 showing novel product. D: CACI-MS1 spectrum of novel product in C showing diagnostic ions for a 20:3 FAME. E: CACI-MS/MS spectrum of the [M+54]+ showing diagnostic ions characteristic of 20:3. Masses of fragments shown in the structural inset indicate the diagnostic ions characteristic of 20:3n-6 (m/z 190 and 262).