Fig. 4.

FA2H silencing facilitates cell growth and inhibits db-cAMP-induced growth arrest in D6P2T cells. D6P2T cells were transfected with control shRNA or FA2H shRNA plasmid and selected for puromycin resistance for 2 weeks. A–D: Cells were treated with sham (A, C) or 0.1 mM db-cAMP (B, D) for 24 h before stained with calcein-AM. E: Cell counts in samples A–D. The counts were normalized against the cell number in A. The mean and SD of triplicate counts are shown. F: [³H]thymidine incorporation. Low confluency cells were exposed to [³H]thymidine for 5 h in a serum-free medium. After extensive washing, cells were lysed to quantify radioactivity by liquid scintillation counting. The mean and SD of sextuplicate measurements are shown.