Figure 1.
DHT, but not estradiol, stimulated basal and Angiotensin II-mediated aldosterone secretion by H295R human adrenocortical cells. H295R cells were incubated with increasing concentrations of DHT (A, C) or 17β-estradiol (B) in the presence or absence of Angiotensin II (10 nM) for 24 h. Aldosterone (A, B) or cortisol (C) concentration was measured in media supernatant and cells lysed to quantify total protein. *: P < 0.05 vs. control cells, #: P < 0.05 vs. basal condition, n = 4.