Figure 2.
DHT-mediated aldosterone secretion is independent of the intra adrenal renin-angiotensin system. H295R cells were treated with 10 μM losartan (A), 10 μM captopril (B) or vehicle for 30 min and then incubated with 1 μM DHT or vehicle in the presence or absence of Angiotensin II (Ang II, 10 nM) for 24 h. Steroid concentration was measured in media supernatant and cells lysed to quantify total protein. *: P < 0.05 vs. no DHT under same conditions, #: P < 0.05 vs. control cells, &: P < 0.005 vs. Ang II treated cells, n = 4.