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. 2009 May 21;4(5):e5654. doi: 10.1371/journal.pone.0005654

Figure 3. Methylation Marks at the H19 Imprinted Locus are disrupted in Germ Cells of Dazl Mutants.

Figure 3

(A) Schematic of expected methylation status of 15 CpG islands in region 1304–1726 of the H19 differentially methylated region (DMR) (GenBank acc. No. U19619) at different time points in the lifecycle of male germ cell development. Open circles denote an unmethylated status of a CpG, while filled circles denote a methylated CpG. (B) Results of bisulphite sequencing displaying an average percent methylation status (y-axis) of 60 clones per datapoint (20 clones per individual sample, 3 replicates for each genotype/age) for both E13.5 and post E16.5 GFP-positive germ cells isolated by FACS. Clones that were hemi-methylated are not shown. ‘***’ denotes a very significant difference (P<0.001) in unmethylated status relative to somatic. ‘*’ denotes a statistically significant difference (P<0.05) in methylated status relative to somatic. ‘‡ ‡ ‡’ denotes an extremely significant difference (P<0.001) in methylated status with E13.5 Dazl+/+. ‘†††’ denotes an extremely significant difference (P<0.001) in methylated status with E13.5 Dazl+/−.