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. 2009 May 21;4(5):e5654. doi: 10.1371/journal.pone.0005654

Figure 5. Dazl is required for Meiotic Progression, but Not for Entry into Meiosis.

Figure 5

Results of analysis of 100 cells/genotype FACS isolated E17.5 Oct4ΔPE:GFP-positive cells, immunostained for SYCP3 (red) to indicate pachytene stage chromosomal alignment of Prophase I, and DAPI (blue) to label the nucleus. (A) Wildtype germ cell in pachytene. (B) Dazl−/− mutant germ cell in late zygotene/early pachytene. (C) Dazl−/− mutant germ cell displaying non-aligned SYCP3 staining. (D) Dazl−/− mutant germ cell displaying no SYCP3 staining. (E) Bar graph displaying percentage of cells per 100 cells/genotype showing appropriate SYCP3 alignment (A, B, red bars), and percentage of cells determined to be ambiguous or not showing SYPC3 alignment (yellow bars).