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. 2009 May 21;4(5):e5654. doi: 10.1371/journal.pone.0005654

Figure 7. Dazl Mutant mESCs Display Aberrant Gene Expression of Pluripotency and Germ Cell Markers During Differentiation.

Figure 7

Gene expression profiles are shown from GFP-positive cells, isolated by FACs, from day 0, 3, 7, 14, 21, 28, and 35 of differentiation. Expression is represented by heat map generated on DChip displaying a continuum with high expression depicted by red to low expression, in blue. The numeric values are from the formula 2∧(−ΔCt), with GAPDH as the housekeeping gene, on a log scale. Genes are classified based by functional group as follows. Group A consisted of the pluripotency related genes Oct4, Sox2, Nanog, and TNAP (Tissue-non-specific alkaline phosphatase). Group B contains epigenetic regulators from the DNA methyltransferase family, Dnmt1, Dnmt3a, and Dnmt3b. Group C consists of the primordial germ cell specific markers Blimp1, Fragilis, Stella, cKit, Pumilio1 (Pum1), Pumilio2 (Pum2), and Nanos3; Group D contains the gonocyte markers Vasa, Stra8, Sycp3 (Synaptonemal Complex Component 3), GCNF, and GDF9. Group E contains the late germ cell markers Tdrd1 (Tudor domain containing 1), Tekt1 (tektin1), and Acrosin; Group F contains pro-apoptotic markers Casp6 (Caspase6) and Bax (BCL2-associated X), as well as the anti-apoptotic marker Bcl-2; Group G contains markers of autophagy including Atg5 (autophagy related 5) and Beclin1.