TABLE 2.
Primers
| Primer | Sequencea |
|---|---|
| 17xxspec_for | GTTACATTTGCATTGCTAGGATCGATTTTCGTTCGTGAATAC |
| 17xxspec_rev | GAGCCTTCAACACAGATTACCATATGCAAGGGTTTATTG |
| 274-lacZ | GGGAAGGGCGATCGGTGCGGG |
| abcB-down_r | ACGGCAGATAATGGAACGGAATTCAATCGC |
| abcB-down_rr | TTCTGAGGAGCACATCTATTATGCGCACCC |
| abcB-downSal_f | tagtgggtcgaCCAAATGTAAAAAAAGATACCTCGACTTCAAAATCGAGG SalI |
| abcB-up_f | CCTTCTTCATAGCTGTTTTACTGGTTATCTTTGGG |
| abcB-up_ff | TGTTTAATGCAGGGATTACCGTTTTCCTCC |
| abcB-upBam_r | cgcggatccTTTA*ATCTAAACCGACTTTCTGCAAGATAATAAAGCGC BamHI |
| aph3Bam_f | cgcggatccAAAGAGGAAGGAAATAATAAATGGCTAAAATGAGAATATCACC BamHI |
| aph3Sal_r | tagtgggtcgaCGAACCAATTGGTTAATTGTAGGCATCTACATTCTCC SalI |
| aphIII_fwd | ATGGCTAAAATGAGAATATCACCGG |
| aphIII_rev | CTAAAACAATTCATCCAGTAAAATATAATA |
| bgaA_rev | GCTAAACCTGCTGCTACTGCTGC |
| cop_up1 | CTCTAATTCCCTTAAAGTGGGAACTAGAG |
| cop_up2 | TTGACTGGACCAGAACCATGACCGAGTTGAG |
| copY_down1 | GAGTTCCCAGCGCAACCAAGGTATCC |
| copY_down2 | CCTGCAACTAACATAATAGGCGTTGTTGC |
| copY_fwd+JanusLin | ACGTCCAAAAGCATAAGGAAAGGGGCCCAAGTAAGATGTAATTGTATGTAAAGGAGACG |
| copY_rev+JanusLin | CGGATCCGATCCATTTCCTCTGGAATAGGCCTGCCATTCTGCATCTGAAATCTGCAT |
| ko-TR_aphIII_fwd | TATTATATTTTACTGGATGAATTGTTTTAGAAAAAGATACCTCGACTTCAAAATCGAGG |
| L17xx_for | TCCAGCATTTCCTACAAGAATAAGTAGAGG |
| L17xx_rev | ATTCACGAACGAAAATCGATCCTAGCAATGCAAATGTAAC |
| Mini-copY_fwd | ATGCAGATTTCAGATGCAGAATGGCAGGAAGTAAGATGTAATTGTATGTAA |
| Mini-copY_rev | TTACATACAATTACATCTTACTTCCTGCCATTCTGCATCTGAAATCTGCAT |
| Proorf1_Kanlink_rev | CCGGTGATATTCTCATTTTAGCCATGATGTTACTCCTTTGTTCTTTATGAGTC |
| Pspr0812_fwd1 | gacgcatgcCTTATATTTTACAACTTTAAAAATAGG SphI |
| Pspr0812_rev1 | cgcggatccCTTTATGAGTCTAGTTTACATCAAAAAAAG BamHI |
| R17xx_for | ACAATAAACCCTTGCATATGGTAATCTGTGTTGAAGGCTC |
| R17xx_rev | CAGAAGAGTTGATTGACCAGCACTATCAGG |
| RACE-PCR_5 | GATATGCGCGAATTCCTG |
| RTgyrAf | TATCACAGCAGTACGTGATGAG |
| RTgyrAr | GGATAGCGAGCATATTGAAACC |
| RTspr0812_fwd5 | AAGTCGTGGTCAGGTTTAC |
| RTspr0812_rev7 | GCGGAAGCAAGATATTGTC |
| RTspr0813_fwd1 | AAACCGCAAACTCTACTATCC |
| RTspr0813_rev1 | GACGACAAACATACCAAATCC |
| spr0812_down3 | TGCTTGTCTTGACGGATCGAAAGAGTAG |
| spr0812_down4 | TTCTTTCCATCACGGCAGATAATGGAAC |
| spr0812_rev5 | GCGGTGTCAGTTCCATTCAAGTAAACC |
| spr0812_rev6 | GATTTACCAGAACCAGACTCACCC |
| spr0812_up1 | ACAGGCTGTAATTTAGTCGGCAATGTGAAG |
| spr0812_up2 | ATTAATAATATTTCGCCAGCTTCATCC |
a
The nucleotides printed in lowercase letters were added to introduce the underlined cleavage sites for the restriction enzymes indicated next to the sequences. The position of the C1744T exchange introduced into spr0813 by the use of the mutagenic primer abcB-upBam_r is indicated by an asterisk.