TABLE 2.
Primers used in the study
| Function and primer | Nucleotide sequence (5′-3′) | Reference or source |
|---|---|---|
| Gene expression | ||
| mexB1 | ATC CGC CAG ACC ATC GCC A | 27 |
| mexB2 | CAT CAC CAG GAA CAC GAG GAG G | 27 |
| mexC3 | GTA CCG GCG TCA TGC AGG GTT C | 11 |
| mexC4 | TTA CTG TTG CGG CGC AGG TGA CT | 11 |
| mexE4 | CCA GGA CCA GCA CGA ACT TCT TGC | 11 |
| mexE5 | CGA CAA CGC CAA GGG CGA GTT CAC C | 11 |
| mexG1 | GCA ACT GGC TCT GGC TGA CC | 27 |
| mexG2 | ACG GCG GTG GCG ATG TTG AA | 27 |
| mexJ1 | GCC CTG TCC CTG TTT TCC TCC C | 27 |
| mexJ2 | CCT TCT TTA CCC GCT CGC CG | 27 |
| mexV1 | CGT CAG CAG ATC GCC CTT TTC AGC | 42 |
| mexV2 | CGC TTT TCG AGA TGG CCT TGC TGC | 42 |
| mexY1a | TTA CCT CCT CCA GCG GC | 33 |
| mexY1b | GTG AGG CGG GCG TTG TG | 33 |
| uvrD1 | CAC GCC TCG CCC TAC AGC A | 34 |
| uvrD2 | GGA TCT GGA AGT TCT GCT CAG C | 34 |
| Gene inactivationa | ||
| mexBrec1 | CTC GGA TCC GTC GGT GAC TTC CAG GTG TT (BamHI) | This study |
| mexBrec2 | CTC AAG CTT GAA AGG AAC ATC CGG TTG AA (HindIII) | This study |
| mexYb1 | CTC GGA TCC GGT CTA CAC CCT GGT CAT CG (BamHI) | This study |
| mexYb2 | CTC AAG CTT GGC CGA CCT TGA AGT AGA TG (HindIII) | This study |
| Mutagenesis experimentsb | ||
| F29S sup | GCG ATC CGC TCC CTG CCG GTC | This study |
| F29S down | GAC CGG CAG GGA GCG GAT CGC | This study |
| F1018 up | CTG GTA CCG CTG CTC TTC CTG GTG GTC | This study |
| F1018 down | GAC CAC CAG GAA GAG CAG CGG TAC CAG | This study |
a
The restriction sites introduced into primers are underlined in the sequences, with the corresponding endonucleases indicated in parentheses.
b
The nucleotide substitutions introduced into primers are indicated in boldface type in the sequences.