TABLE 3.
Partial protein sequence alignments of antibiotic-resistant and -susceptible isolates
| Isolate tested with indicated antibiotica | Sequenceb |
|---|---|
| Erythromycin | |
| L4 isolates | |
| SV3s | 63KPWRQKHT70 |
| UHWO10r | 63KPW--KHT70 |
| Tetracycline | |
| TetM isolates | |
| SV9 Seattler | 209HNCSLFPVYHGSAKNNIGID228 |
| HPA23r | 209HNCSLFPVYHGSAKNNIGID228 |
| HPA6s | 209QNCSLFPLYHGSAKSNIGID228 |
| SV9 Vancouverr | 209QNCSLFPLYHGSAKSNIGID228 |
| Fluoroquinolone | |
| ParC isolates | |
| Consensuss | 78HPHGDSSIYE87 |
| HPA18r | 78HPHGNSSIYE87 |
| Bebear UUg2-5r | 78HPHGDLSIYE87 |
| Bebear UUcr | 78HPHGDSSIYK87 |
a
Superscript letters (r and s) indicate resistance and susceptibility, respectively, to the indicated antibiotics.
b
Boldfaced amino acids identify areas of sequence divergence within a variable region of the protein. The sequence of TetM of the Vancouver strain was determined at the UHW. Underlined amino acids identify areas of sequence divergence relative to the sequences of susceptible strains. Previous S83L amino acid substitutions arising from mutation and E87K substitutions associated with resistance as described by Bebear et al. (2) are also shown. -, deletion of amino acids R and Q.