TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Phenotype, genotype, and/or characteristicsa | Reference or source (year of isolation) |
|---|---|---|
| Strains | ||
| S. enterica serotype Westhampton | ||
| 04CEB8118SAL | ESBL, blaCTX-M-53 | Living cockles, fishery, Morbihan, Franceb (2004) |
| 04CEB8273SAL | ESBL, blaCTX-M-53 | Living cockles, supermarket, Puy-de-Dôme, Franceb (2004) |
| 04CEB8276SAL | ESBL, blaCTX-M-53 | Living cockles, supermarket, Vienne, Franceb (2004) |
| S. enterica serotype Senftenberg | ||
| 04CEB8275SAL | ESBL, blaCTX-M-53 | Living cockles, supermarket, Vienne, Franceb (2004) |
| E. coli | ||
| ATCC 25922 | Control in disk diffusion method and in MIC determinations | CDC, Atlanta, GA |
| DH1 | F−endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 | |
| DH5α | DH1 genotype and F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 | |
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 endA1 araΔ139 Δ(ara, leu)7697 galU galK-rpsL nupG tonA | Invitrogen |
| HB101 | hsd20(rB− mB−) recA13 rpsL20(Strr) leu proA2 | |
| Plasmids | ||
| pBK-CMV phagemid | Neor Kanr | Boehringer-Mannheim |
| pOX38-Neor | F derivative Tra+ Neor, 58.2 kb | 18 |
| pGBG1 | pBBR1 derivative Chlr, 7.6 kb | 35 |
| pWES-1 | 11-kb natural plasmid from S. enterica 04CEB8273SAL electrotransformed in E. coli DH10B | This study |
| pCTX-M-53 | 1.5-kb PCR fragment from S. enterica 04CEB8273SAL that contained blaCTX-M-53 in the XbaI-PstI sites of pBK-CMV | This study |
a
Strr, streptomycin resistant; Neor, neomycin resistant; Kanr, kanamycin resistant; Chlr, chloramphenicol resistant.
b
Département, French administrative subdivision.