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. 2009 Mar 4;16(5):621–627. doi: 10.1128/CVI.00474-08

FIG. 2.

FIG. 2.

Improving the performance of the LIPS test. The 83 sera of the training set, 43 controls (CTRL) and 40 KS/HHV-8-infected subjects (KS), were reblinded and retested with a new five-antigen mixture employing K8.1-Δ4 instead of K8.1-Δ1 and K8.1-Δ2 (A), as well as with K8.1-Δ4 alone (B). Use of the same cutoff of 9,000 LU with the new five-antigen mixture resulted in improved diagnostic performance of 95% (38/40) sensitivity and 93% (40/43) specificity. The sum of antibody titers to only four antigens (LANA-Δ2, K8.1-Δ4, v-cyclin, and ORF65) was recalculated (C), and using the 9,000-LU cutoff (solid lines) with these four antigens markedly improved the test (95% sensitivity and 100% specificity). A volunteer sample (non-KS) from the training cohort that was found HHV-8 positive by both ELISA and LIPS is included with the KS samples.