FIG. 3.

A four-HHV-8-antigen panel shows 100% sensitivity and 100% specificity. Antibodies were evaluated by LIPS with a 71-serum validation cohort, 36 controls (CTRL), and 35 KS/HHV-8-infected subjects (KS), using a four-antigen panel consisting of K8.1-Δ4, LANA-Δ2, v-cyclin, and ORF65. These LIPS tests were either evaluated separately and summed (A) or tested as a cocktail (B). As shown, the two tests showed similar results and had 100% sensitivity and specificity. The 9,000-LU cutoff is shown by the solid lines. A volunteer sample (non-KS) from the validation cohort that was found HHV-8 positive by both ELISA and LIPS is denoted by the triangles.