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. 2009 Mar 25;16(5):739–748. doi: 10.1128/CVI.00478-08

FIG. 5.

FIG. 5.

Specificity of the IsdB serologic assay. A. Adsorption of IsdB-specific IgG antibodies with yeast-derived IsdB or VLP16. Sixteen sera with low to high IsdB-specific antibody titers were mock adsorbed with PBS or adsorbed with 3 μg of HPV VLP 16 or 3 μg of IsdB/y and tested in the IsdB serologic assay at a dilution of 1:2,500. B. Analytical specificity was determined in the IsdB serologic assay by calculating the percentage of antibody adsorbed with either HPV VLP16 or IsdB/y compared to PBS. The dashed line represents 85% specificity. The overall specificity of the IsdB serologic assay is 97.8%.