TABLE 2.

Sensitivity and specificity of the b-ELISA using different diagnostic criteria^a

MAbb	Method of calculation of cutoff valuesc	Cutoff valued	% Overall agreement with PRNT90	Sensitivity (%)	Specificity (%)
3.1112G	2 SD	0.278	79	73	100
	3 SD	0.245	70	63	95
	ROC	8%	91	89	92
	ROC	30%	70	63	95
6B6C-1	2 SD	0.254	70	63	97
	3 SD	0.192	56	45	100
	ROC	13%	79	75	93
	ROC	30%	64	57	97

^aDiagnostic results using the b-ELISA for the detection of antibodies to WNV and other flaviviruses are shown for 366 human serum samples from Colorado. Results were compared with PRNT₉₀ values, the gold standard for WNV diagnosis.

^bThe MAb 3.1112G is WNV specific and the MAb 6B6C-1 is flavivirus specific.

^cCutoff based on 2 standard deviations for the noninfected reference population based on optical densities obtained with the b-ELISA; ROC, based on receiver operating characteristic curve analysis.

^dCutoff values were determined by the different methods described above and are shown as percentages for the ROC analyses.