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Immunoblot assay of GST-Hag recombinant proteins. Purified polypeptides were resolved by SDS-PAGE, transferred to polyvinylidene difluoride, and probed with an anti-GST antibody. Construct names are shown at the bottom. Molecular mass markers are noted on the left. Refer to Fig. 5 for details regarding what portion of O35E-Hag MID is specified by each construct.
