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. 2009 Mar 25;16(5):653–659. doi: 10.1128/CVI.00460-08

FIG. 5.

FIG. 5.

Results of immunoassays with eight recombinant peptide constructs of Hag/MID. The black bars on the left indicate the amino acid numbers that comprise each of the GST-tagged recombinant proteins. Construct names are shown to the left of each bar. The right side indicates results of immunoblot assays with each of the constructs assayed for serum IgG (top) and sputum IgA (bottom) from adults with COPD who acquired and cleared M. catarrhalis. A + indicates reactivity in the immunoblot assay, and an empty box indicates absence of reactivity. The numbers at the bottom of the serum IgG panel indicate the results with the following serum samples: 1, 10E67; 2, 19E55; 3, 32E8; 4, 39E34; 5, 11E31; 6, 46E49; 7, 52E4; 8, 63E25; 9, 87E16. The numbers at the bottom of the sputum IgA panel indicate results with IgA purified from sputum supernatants: 1, 3PS68; 2, 12PS75; 3, 7PS95; 4, 10PS92; 5, 19PS55; 6, 66PS6; 7, 29PS25; 8, 44PS24; 9, 1PS45. The percent identities of Hag/MID among seven strains whose hag gene has been sequenced are noted on the right.